Fig. 5.

G4 flow cytometry analysis of mouse macrophage cells. a Histogram plot of the FL1-BG4 signal distribution in mouse macrophages incubated with BG4 (black) or without antibody (white). b Labeling of untreated mouse macrophages or cells incubated 24 h with 7 μM or 25 μM PDS with the BG4 antibody (white). Nucleus border is defined by the white line. Scale bar 10 μm. Below G4 levels determined by fluorescence intensity of nucleus normalized over untreated. Plotted results were based on the average of at least n = 3 biologically independent experiments. The horizontal gray line represents the mean value. Significance was determined using an ordinary one-way ANOVA multiple comparison: ****p < 0.0001. c Quantification of the FL1-BG4 signal in untreated mouse macrophages or cells incubated 4 h with 25 μM PDS. Plotted results were based on the average of n = 3 biologically independent experiments. Significance was calculated based on a one-sided Student’s t-test. d Histogram plot of the FL1-BG4 signal distribution in untreated mouse macrophages (white) or cells incubated 4 h with 25 μM PDS (black). Results are based on the average of at least n = 3 biologically independent experiments. Significance was calculated based on a one-sided Student’s t-test. Asterisks indicate statistical significance in comparison with wildtype cells: **p < 0.01. An excel file providing individual data values is available in the section “Availability of data and materials”