Fig. 2

Hierarchical clustering of Leptosphaeria maculans gene expression in 32 conditions representing the fungal life cycle. Each gene with an FPKM count > 2 in at least one set of conditions was retained for the correlation analysis. A pairwise Pearson’s correlation analysis was performed between the 32 sets of conditions on Log₂(FPKM+ 1) values. The resulting Pearson’s correlation matrix was subjected to hierarchical clustering (represented by the tree) with the ward.D2 method. The Pearson’s correlation results are shown on a color scale. Four groups of clustered samples were identified (GP1 to GP4). The experimental conditions are indicated as IPF, in planta field conditions; IPC, in planta controlled conditions; IV, in vitro conditions and RF, residues in field conditions. The infected plant tissues collected or the conditions under which the in vitro samples were obtained are detailed, together with the corresponding time points for sampling (DPI, days post inoculation; MPS, months post sowing; MPH, months post harvest; d, days; h, hours)