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Fig. 3 | BMC Biology

Fig. 3

From: Sea urchin larvae utilize light for regulating the pyloric opening

Fig. 3

The 5HT2 receptor mediates the light>pylorus signaling pathway. a The opening rate of the pylorus under photoirradiation was extremely reduced by the addition of melperone hydrochloride and ketanserin tartrate (5-HT2 receptor antagonists). N = 4; n (control) = 54, 26, 42, 66; n (melperone hydrochloride) = 15, 17, 27, 36; n (ketanserin tartrate) = 17, 17, 27, 25. Error bars show SE. b The putative cis-regulatory elements of the 5HT2 receptor drove Venus signaling in the stomach. The rate of Venus expression in the stomach was 81.4% (57/70) in all larvae that had Venus signals. s, stomach; i, intestine. c The activity of alkaline phosphatase in the gut was invariant in control and 5HT2 morphants. d The graph shows that the 5HT2 receptor was required for the light>pylorus signaling pathway. N = 2–3; n (control with no treatment) = 25, 37; n (control with photoirradiation) = 21, 11, 55; n (control with serotonin) = 25, 19, 22; n (control with SNAP) = 28, 14, 19; n (5HT2 morphants with no treatment) = 13, 16; n (5HT2 morphants with photoirradiation) = 16, 16, 17; n (5HT2 morphants with serotonin) = 35, 45, 23; n (5HT2 morphants with SNAP) = 28, 28, 37. Error bars show SE. Scale bars in b and c = 50 μm

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