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Fig. 7 | BMC Biology

Fig. 7

From: Evolution of the codling moth pheromone via an ancient gene duplication

Fig. 7

Functional characterization of desaturase activity of Cpo_CPRQ in insect cells. Total ion chromatograms of methyl esters (FAME) samples from Sf9 cells supplemented with lauric methyl ester (C12) infected with a empty virus (control) or b recombinant baculovirus expressing Cpo_CPRQ. Cpo_CPRQ produces large amount of E9-12:Me and E8E10-12:Me. Sf9 insect cells infected with bacmid expressing Cpo_CPRQ in the medium in the presence of the monoenic intermediate c (E)-9-dodecenoic methyl ester (E9-12:Me) or d (Z)-9-dodecenoic methyl ester (Z9-12:Me). The retention time and mass spectrum of the E8E10-12:Me peaks observed after addition of 12:Me and E9-12:Me were identical with those of the synthetic standard. The relatively long retention time (compound eluting later than 14:Me) is in agreement with what is expected from a diene with conjugated double bonds. e Spectrum of the E8E10-12:Me peak in b. The relatively abundant molecular ion m/z 210 is in agreement with the expectation for a diene with conjugated double bonds. f Analyses of MTAD-derivatized samples displayed diagnostic ions at m/z 323 (M+), m/z 308, and m/z 180 (base peak), confirming the identification of the conjugated double-bond system

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