Fig. 2

Chronic heat stress causes yolk protein accumulation and mislocalization. a A schematic showing the relationship between the six vit genes, their initial protein products, and the three major yolk protein (YP) bands. b Coomassie-stained SDS-PAGE reveals accumulation of all three major YP species following chronic exposure to 28 °C HS. Arrows indicate YP170, YP115, and YP88 bands. The rme-4(b1001) mutant YP170::GFP strain (RT362) was included for YP band identification. Shown is a representative image from three independent trials. MW: molecular weight marker; WT: wild-type. c–h Chronic exposure to 28 °C HS causes accumulation and mislocalization of the YP170::GFP (RT130) fluorescent reporter. Arrows in c, e, and g demonstrate proper localization of YP170::GFP in embryos at the control temperature. Shown are representative images from three independent trials with n ≥ 10 worms in each trial. Scale bar: 150 μm