Fig. 3

Chronic heat stress inhibits endocytosis in coelomocytes and disrupts neuronal signaling. a–d Chronic exposure to 28 °C HS (b and d) disrupts coelomocyte endocytosis compared to 20 °C controls (a and c) as observed in myo-3p::ssGFP (GS1912) adults. Shown are representative images from three independent trials with n ≥ 10 worms. Arrows in a and c demonstrate proper localization of ssGFP in coelomocytes at the control temperature. Scale bar: 150 μm. e A schematic showing the cycle of synaptic vesicle formation and neurotransmitter release at a neuromuscular junction. Synaptic vesicle components are normally recycled through endocytosis (dashed arrows). f Exposure of WT (N2) adults to 48-h 28 °C HS increases resistance to aldicarb, but not to levamisole, compared to 20 °C controls as measured using paralysis assays. Data represent the mean ± SD of n = 3 independent trials. * p < 0.05 (Student’s t test)