Fig. 4

hsp-1 knockdown inhibits yolk endocytosis. hsp-1 knockdown causes yolk to accumulate in the pseudocoelomic space, as shown in YP170::GFP (RT130) worms that were raised to the L4 stage on OP50 and then exposed to either a control (L4440, empty vector) or b hsp-1 RNAi for 48 h at 20 °C. Shown are representative images from three independent trials with n ≥ 10 worms. Scale bar: 150 μm