Fig. 3

Analysis of the reproductive sex ratio in Cas9 and Cas12a transgenic strains. a Percentage of adult males in the progeny of transgenic males (blue) or females (red) from each transgenic strain crossed to the wild type. Each strain was assayed over for four consecutive generations. b Egg-to-adult survival rate of the progeny of transgenic males crossed to wild-type females of the Cas9.1 and Cas9.2 transgenic strains compared to the wild type. c Relative expression of Cas9 determined by quantitative PCR in different tissues for the hemizygous transgenic strains Cas9.2d (2d), Cas9.2c (2c), Cas9.2b (2b), Cas9.2a (2a), and Cas9.1e (1e). d A wild-type male (WT), a Cas9.2a hemizygous transgenic male carrying a single autosomal transgene (2a) and a Cas9.2a/Cas9.2c transhemizygote male carrying two autosomal transgenes (2a2c) observed using the RFP filter (top panel) and cold light source (bottom panel). e Percentage of males in the progeny of males carrying the Cas9.2a (2a), Cas9.2a/Cas9.2a (2a2a), Cas9.2a/Cas9.2c (2a2c), Cas9.2c/Cas9.2c (2c2c), Cas9.1e/Cas9.1e (1e1e) and Cas9.1e/Cas9.2c (1e2c) transgenes crossed to wild-type females, compared to a wild type cross (WT). The numbers indicate the total number of individuals scored. ns p > 0.05, *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0001