Fig. 1
From: Natural abundance isotope ratios to differentiate sources of carbon used during tumor growth in vivo
C3- and C4-plant based fly food for stable isotopic labeling of D. melanogaster. Food composition (a) and δ13C of the food components and flies developed on the indicated food types (b). Individual food components in b are measured in duplicate or singlicate (for C4 yeast). The C3 and C4 composite food types are measured with six replicates and the larvae growing on the two food types are measured with 23 biological replicates. c 13C/12C measurements of the cephalic complex (eye disc and brain) from fly larvae with genetically programmed (RasV12,scrib−/−) tumors in the eye disc. Quantified as δ13C units per mil (‰), relative to the international 13C/12C standard Vienna Peedee Belemnite (VPDB). “Host tissues” is a measurement of the remaining organs of the larvae after removing the cephalic complex. Measurements of the food used in these experiments are also included. The larvae organ measurements in c are from three biological replicates for each indicated time of larvae development. Each datapoint represents the SIRMS measurement of a single animal. Box plot are used for visualizing the data with default settings for geom_boxplot in R; the median as a line inside boxes extending from the 25th percentile to the 75th percentile and whiskers extend maximally to 1.5x of the inter-quartile range