Fig. 4.

Missexpression of active Notch (aN2) in quail neural tubes prevents the upregulation of RP markers. Left column represents electroporation of control GFP (a, g, m, s) (N = 7 for Bambi, and N = 3 for Rspo1, Raldh2, and Draxin) or aN2-GFP (d, j, p, v) (N = 7,15,6,6 for Rspo1, Bambi, Raldh2, and Draxin, respectively). Middle column depicts ISH for the denoted genes in control GFP (b, h, n, t) or aN2 (e, k, q, w), and right column is an overlay of the precedent, respectively. Note that control GFP-treated cells co-express both GFP and the RP genes (arrowheads), whereas aN2-treated cells are devoid of marker expression (arrows). Bar = 50 μm