Fig. 5

MiR827 and GPLα expression is induced following exposure to Pi-deficient growth conditions and GPLα is localized to Arabidopsis cell nuclei. A and B Measurement of miR827 (A) and GPLα (B) expression in either Col-0 (Col) or Ler wild type 10-day-old seedlings following an additional 10 days under Pi-deficient growth conditions. Asterisks indicate significant differences (P < 0.05, Student’s t test, ±SD). C and D GFP fluorescence imaging in leaves and roots of transgenic plants (Col-0 background) containing miR827-promoter-driven GFP (miR827Pro:GFP) (C) or GPLα-promoter-driven GFP (GPLα Pro:GFP) (D) under Pi deficiency (upper panels) or optimal Pi (lower panels). Six independent transgenic lines were examined. Representative lines are shown. E and F Arabidopsis wild type (Col-0) leaves were transiently transformed with a vector including the control GFP construct, 35S:GFP (E) or a translational fusion between GPLα and GFP, 35S:GFP::GPLα (F). GFP protein localization was visualized using confocal microscopy. (a) GFP fluorescence; (b) chlorophyll autofluorescence; (c) bright-field image; (d) overlay of a, b, and c