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Fig. 10 | BMC Biology

Fig. 10

From: Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility

Fig. 10

The positions of the rat meiotic DSB hotspots are determined by PRDM9. Left, a region of rat Chromosome 17 (rn5 assembly) exemplifying that the DSB hotspots (shown as peaks) in wild-type SHR males (SHR Prdm9+/+ SSDS) are shared with the strain with the same Prdm9 allele (WKY Prdm9+/+ SSDS), but not with the strain with different Prdm9 allele (BN/RIJHsd Prdm9+/+ SSDS) or inactivated Prdm9 allele (SHR Prdm9−/− SSDS). DSB hotspots in Prdm9-deficient male co-localize with testicular (T) and less with liver (L) H3K4me3 marks. The H3K4me3 profiles from the BN a WKY strains are different and reflect hotspot sites unique to each of these strains, thus validating that rat PRDM9 displays H3-methyltransferase activity. Some promoters correspond to Prdm9-independent hotspots (green arrowheads), but other promoters (pink arrowheads) are not targeted. The row “Genes” shows Ensembl gene models. Raw ChIP-seq coverage is shown in 150-bp windows; each panel is scaled to the maximum value. Right, DNA-binding zinc-finger arrays of PRDM9 from two rat strains showing polymorphic amino acid residues in contact with DNA

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