Fig. 2

Decreased fertility parameters of SHR males carrying Prdm9 deletions depend on age. Each dot depicts a single animal. a–c Boxplots of sperm count (a), testicular weight (b), and relative testicular weight (c, mg testicular weight per gram of body weight). d, Representative images of hematoxylin-eosin (HE) stained sections from rat testicles; note the presence of mature spermatids (arrows) in all three males. e Litter size of male parents of two indicated Prdm9 genotypes for paternal age at birth. The statistical significance of the differences between genotypes was tested using LRM in the age groups of up to 150 and above 150 dpp. f Number of pups per month per female (OMU) for mating ranges (days) in SHR males of two indicated Prdm9 genotypes. The data underlying all published plots are given in Additional file 3