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Fig. 4 | BMC Biology

Fig. 4

From: Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility

Fig. 4

Prdm9-deficiency causes spermatogenic arrests in tubular stages IV and XIV of SHR rat. a Testicular histology using PAS-H staining. Illustrative examples of all 14 rat tubule stages. Degenerated cells in seminiferous tubules from adult (71 dpp) mutants stain intensively with PAS-H (blue-purple) at stages IV and XIV (red arrows). P, pachytene spermatocytes; RSP, round spermatids; ESP, elongated spermatids; S, Sertoli cells; Pl, preleptotene; L, leptotene; D, diplotene; Z-P, zygotene-pachytene spermatocytes; MI, metaphase I spermatocytes. Quantification of cells in all stages is presented in Fig. 5. b Increased apoptosis in mutant rat stage IV and XIV tubules confirmed by labeling apoptosis (TUNEL), acrosome (PNA), and DNA (Hoechst 33342)

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