Fig. 5

HS-induced cell death response is not ferroptosis. a SO staining of BY-2 cell cultures demonstrates that pre-treatment with 1 μM Fer-1 does not affect HS-induced cell death. Staining for no HS and 55 °C treatments was performed according to protocols i and iii, respectively (see “Sytox Orange and FM4-64 staining”). b Quantification of cell death frequency in the samples illustrated in a. The chart shows representative results of three independent experiments, each including ≥ 280 cells per treatment and time point. c Pre-treatment with Fer-1 does not alleviate the HS-induced death of Arabidopsis thaliana root hair cells. Arrows indicate SO-positive nuclei. Three independent experiments demonstrated the same results. No quantification was performed in these experiments, since all cells were SO-positive. DIC, differential interference contrast. Scale bars, 20 μm