Fig. 2From: Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particlesPurity of DI244 material analyzed for other defective vRNAs. DI244 produced at different MODIPs was analyzed by segment-specific reverse transcriptase-PCR for the presence of short vRNAs in Seg1–8. Results for a MODIP 1E−1, b MODIP 1E−2, c MODIP 1E−3, and d MODIP 1E−4 are shown. Signals corresponding to full-length (FL) and deleted (DL) vRNAs are indicated (FL size depends on the analyzed vRNA segment). Ladder: upper thick band 3.0 kb, middle thick band 1.0 kb, lower thick band 0.5 kbBack to article page