Fig. 7

D2-Mx1^−/− infections with DI244 material. Female 8–12 weeks old D2-Mx1^−/− mice were intra-nasally infected with 20 μL solution containing 10 μL of the tested substance and 1000 FFU PR8 STV in PBS at day 0. Toxicity was tested by applying DI244 material produced in a shake flask (active, 1.5E+6 PFU per mouse; or UV-inactivated). Active DI244 (n = 5): D2-Mx1^−/− mice treated with DI244 only; inactive DI244 (n = 5): D2-Mx1^−/− mice treated with UV-inactivated DI244 only; STV (n = 10): D2-Mx1^−/− mice infected with PR8 STV; STV + active DI244 (n = 11): D2-Mx1^−/− mice infected with PR8 STV and treated with active DI244; STV + inactive DI244 (n = 10): D2-Mx1^−/− mice infected with PR8 STV and treated with UV-inactivated DI244. a Body weight loss curves of treated mice in percent body weight relative to day 0. Error bars indicate the standard error of the mean (SEM) for body weight changes. b Kaplan-Meyer survival curves. The survival rate for mice treated with active or inactive DI244 alone was 100%. Survival of mice treated only with DI244 was significantly (log rank test; p < 0.01) different from infected mice. Survival rates of mice treated with active DI244 versus UV-inactivated DI244 were not significantly different