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Fig. 5 | BMC Biology

Fig. 5

From: Measuring and modeling energy and power consumption in living microbial cells with a synthetic ATP reporter

Fig. 5

Workflow of kinetic model development. a Black arrows correspond to glucose fluxes converting glucose to biomass, carbon dioxide and water (respiration), or to the byproduct acetate, the excretion flux. Similarly, blue arrows correspond to acetate fluxes converting acetate to biomass or to carbon dioxide and water. The stoichiometry is based on general biochemical processes for glucose and acetate metabolism. Acetate utilization only occurs after glucose is depleted. The mathematical terms on each arrow describe the flux, or consumption rate, for that path in (M/s). Given the glucose or acetate consumption rate for each pathway, the total ATP production rate can then be calculated via stoichiometry (mole product per mole substrate consumed). Biomass production is based on the carbon balance between the substrate consumed and cellular carbon produced (more details are provided in the “Methods” section). b All ATP production fluxes are gathered and converted to the cellular ATP pool. This pool is simultaneously drained for bacterial growth and for cell maintenance via the mathematical flux rate terms (M/s) as indicated in the figure

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