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Fig. 5 | BMC Biology

Fig. 5

From: TORC1 regulates the transcriptional response to glucose and developmental cycle via the Tap42-Sit4-Rrd1/2 pathway in Saccharomyces cerevisiae

Fig. 5

TORC1 regulates the expression of glucose-responsive genes independently of Sch9. Wild type and sch9Δ cells were grown to logarithmic phase in SC/EG medium and then glucose (2% final concentration) was added to the cultures in the presence of either rapamycin (200 nM) or DMSO. Aliquots of the cultures were taken after 0’, 30’, 60’, and 120’. RNA was extracted from the cultures and the expression of the indicated 7 TGC genes (GFD2, GPG1, UGA1, RME1, CIT1, CRC1, and DHR2) were analyzed by real-time qRT-PCR. Data are presented as means ± standard deviation (n = 2 technical replicates). Data from two additional biological replicates of this experiment are presented in Fig. S7

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