Fig. 7

TORC1 regulates the glucose-responsive genes via Sit4 and Rrd1/Rrd2 proteins. Wild type, sit4Δ, rrd1Δ, rrd2Δ, and rrd1Δ rrd2Δ cells were grown to logarithmic phase in YPD medium and then treated with either rapamycin (200 nM) or DMSO. Aliquots of the cultures were taken after 0’, 30’, and 60’. RNA was extracted from the cultures, and the expression of the indicated 7 TGC genes was analyzed by real-time qRT-PCR. D1/R1 and D2/R2 indicate DMSO-treated/rapamycin-treated cells after 30’ and 60’ respectively, and the expression fold-change values were normalized with respect to DMSO-treated cells at t = 0’. Data are presented as means ± standard deviation (n = 2 technical replicates). Data from two additional biological replicates of this experiment are presented in Fig. S10