Fig. 8

TORC1 regulates transcriptomic changes during spore germination. a Wild type spores and gtr1Δ spores were transferred into YPD medium in the presence of either DMSO or rapamycin (2 μM). Activity of TORC1 was assayed by monitoring Sch9 phosphorylation using an anti-HA antibody. b Experimental outline to analyze the role of TORC1 in spore germination by RNA-Seq. Spores were transferred to YPD medium containing either DMSO or rapamycin (2 μM). Aliquots of yeast cells were taken at the indicated time points (0’ 10’, 30’, 60’, 120’, 240’, and 360’) from the two cultures and used for preparing RNA for RNA-Seq analysis. c Transcriptomes of spores treated with rapamycin (2 μM) after 30’ and 60’ were compared with the transcriptomes of corresponding DMSO-treated spores. logFC (fold-change) was plotted against false discovery rate (FDR). Differentially expressed genes (at least 2-fold difference in comparison to DMSO-treated spores) were identified. Genes positively regulated and negatively regulated by TORC1 are indicated by green and red dots respectively and their numbers are indicated at the top of the plot