Fig. 9

TORC1 activity is required for spore germination. a Images showing the morphological changes occurring during spore germination along with their corresponding timing of appearance above. A distinct constriction seen in the germinating spores after 4–5 h following transfer to YPD medium is indicated by the black arrowhead. b Wild type and TOR1-1 spores were transferred into YPD medium in the presence of either DMSO or rapamycin (2 μM). Progress of spore germination was assayed by scoring fraction of cells with different morphologies at the indicated time points for 8 h following transfer into YPD medium. c Wild type spores were transferred into nutrient medium in the presence of either DMSO or rapamycin (2 μM). DNA content of germinating spores following 5 h, 6 h, 7 h, and 8 h following transfer to YPD medium was assayed by flow cytometry. Propidium iodide signals of a haploid and diploid strain are indicated for reference