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Fig. 1. | BMC Biology

Fig. 1.

From: Parental energy-sensing pathways control intergenerational offspring sex determination in the nematode Auanema freiburgensis

Fig. 1.

Dauer and hermaphrodite development are induced across generations in A. freiburgensis. a When hermaphrodite mothers are cultured in non-crowding conditions, in the absence of conditioned medium (CM), most of the XX F1s are female (N = 10 broods, from which a total of 149 F1s were scored). When mothers are in CM of crowded cultures, most of the XX F1s are hermaphrodites (N = 10 broods, with a total of 199 F1s scored). The data in colored dots represent the percentage of F1 hermaphrodites in each brood and is plotted on the upper axes. The colored vertical lines indicate ± SD, and the mean is represented as a gap in the lines. b In C. elegans, the L1 larvae respond to environmental signals to facultatively form stress-resistant dauers. In A. freiburgensis, it is the mother and not the L1s that respond to environmental signals. A. freiburgensis dauers obligatorily develop into hermaphrodite adults. c In the experimental setup (top), the same individual mother hermaphrodite was transferred every 24 h to a new environmental condition. Initially, it was placed in a plate without conditioned medium (−) CM, followed by the transfer to a (+) CM plate and then to a new (−) CM plate. The plot representation is the same as for Fig. 1a. On the last day, 5 mothers died and thus only 9 broods were scored

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