AMPK pathway modulation in the A. freiburgensis germline. a Mean percentage of hermaphrodite and female F1 offspring from hermaphrodites treated with chemicals. The control was either water (left) or DMSO (right), depending on how the chemical compounds were dissolved. Error bars represent SD. Dors. = Dorsomorphin. In all cases, diluted (1:10) CM was added to the medium, with exception to plates with dorsomorphin, which had undiluted CM. b, c Mean antibody fluorescence () in the premeiotic (blue) and meiotic portion (red) of the germline, in the absence (−) or presence (+) of conditioned medium. N = sample sizes. The mean difference for the two comparisons is shown as a Gardman-Altman estimation plot. The raw data is plotted on the upper axes, with colored vertical lines indicating ± 95% CI, and the mean is represented as a gap in the lines. Each difference of the means is plotted on the lower axes as a bootstrap sampling distribution. The difference of the means is depicted as a black dot and 95% confidence intervals are indicated by the black vertical error bars. n.s., p > 0.05; *** = p ≤ 0.01. d Western blots with proteins derived from hermaphrodites incubated in the absence (−) CM or presence (+) CM of conditioned medium. e AMPK pThr172 antibody staining of gonads dissected from hermaphrodites incubated in either (−) CM or (+) CM. Bar, 15 μm. Insert in the right picture is a magnification from the region marked with a stippled square. Bar, 7.5 μm. Details for the one-way analysis of variance (ANOVA) followed by a post hoc Holm-Sidak test for multiple comparisons (a), *** = p ≤ 0.01. See “Methods” for sample sizes and p values.