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Fig. 3. | BMC Biology

Fig. 3.

From: Parental energy-sensing pathways control intergenerational offspring sex determination in the nematode Auanema freiburgensis

Fig. 3.

mTOR signaling modulates intergenerational inheritance in A. freiburgensis. a Mean percentage and SD of hermaphrodite and female F1 offspring from hermaphrodites incubated with either DMSO or pharmacological compounds, together with some CM (1:10) CM. One-way analysis of variance (ANOVA). *p ≤ 0.05, **p ≤ 0.01. b–d Staining with S6K pThr389 antibody (red) and DAPI (blue) of dissected gonads from hermaphrodites incubated either in the absence ((−) CM) or in the presence ((+) CM) of conditioned medium. The arrows indicate cells marked with the antibody in the premeiotic region (b) and in the meiotic region (c), respectively. In d, only the rachis has staining. Bar, 15 μm. e Percentages of gonads with signal for S6K pThr389 antibody staining. The different colors represent the percentage of gonads with at 0, 1, 2, 3, or more than 3 cells stained in the premeiotic tip. Quantification was performed from gonads isolated from animals in the absence (−) or in the presence (+) of conditioned medium, and in the presence of metformin (M). The number of gonads analyzed is indicated on the top of the bars

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