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Fig. 4 | BMC Biology

Fig. 4

From: The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress

Fig. 4

Comparison of tolerance to drought stress, population differentiation, and nucleotide diversity ratio among different M. ruthenica accessions. a The geographic sites for collection of 20 M. ruthenica accessions. b Survival rates of 20 M. ruthenica accessions at the twelfth day exposed to drought stress. Four biological replications and 15 seedlings in each biological replication were used to determine survival rate. All the survival rates under control are 100%. Different letters mean significant differences among treatments at P<0.05. Data are means±SE (n=4). c Correlations between survival rates under drought stress and annual precipitation of sites at which the M. ruthenica seeds were collected. d Phenotypes of two accessions collected from sites of Zhenglanqi and Xinghe. The phenotypes were photographed at the twelfth day of drought. e Neighbor-joining tree of M. ruthenica accessions. Mt J.A17: M. truncatula Jemolong A17. f Population differentiation (Fst). g Nucleotide diversity ratio (log2(θπControl/θπTolerance)) between tolerance and control population. Points above lines are 5% of the biggest Fst and log2(θπControl/θπTolerance), respectively. Points of log2(θπControl/θπTolerance) less than zero are not shown

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