Fig. 5

Interaction of Cox13 with ATP. a CSPs of Cox13 upon ATP addition, for ¹⁵N-HSQC spectra, see Additional file 1: Figure S8. CSPs as a function of primary sequence calculated from peak positions in ¹⁵N-HSQCs at the initial and final titration steps of [ATP]/[Cox13]=0 and 100, respectively (left). The residues with the largest CSPs are indicated as sticks on a representative structure from the NMR ensemble (right). b CSP as a function of ATP/Cox13-concentration ratio for the residues highlighted in a. The curves are in the form of Eq. 6 in [39] with globally fitted K_d = 15±2 mM. The fitted maximum CSPs for residues R81, H83, Y96, and I125 are 0.55±0.04, 0.29±0.02, 0.18±0.02, and 0.29±0.02 ppm, respectively. c Part of ³¹P spectra showing signals from the three phosphate groups of ATP in solutions with (upper panels) and without Cox13 (lower panels) at the concentration ratios given in the legend. The lower panels were obtained on a sample where ATP stock solution was added in the corresponding amounts to NMR buffer only. To ease shift change comparisons between signals from spectra with large ATP concentration differences, spectra have been normalized to the same P^α peak intensity