Fig. 3.
DeProj. aDeProj is the second component of the toolbox and works by combining the tissue surface obtained e.g. via the height-map (gray line) and the segmentation of cells obtained on the 2D projection (green) to make measurements on the real cell contour in 3D (red). b Example DeProj outputs, from left to right and top to bottom: the local mean curvature of the epithelium experienced by the cells; a 3D visualization of the epithelium 2D projection mapped on its 3D surface; the cell eccentricity measured in their oblique apical surface plane; the error (1−l2D/l3D) comparing the cell perimeter measured on the 2D projection versus its real value inferred by DeProj. Scale bar: 10 μm