Fig. 5From: Characterization of the dynamics and variability of neuronal subtype responses during growth, degrowth, and regeneration of Nematostella vectensisDifferential regenerative responses of longitudinal neurons are partially dependent on the size of the remnant fragment. a, b Schematics demonstrating the shifts in amputation sites used to obtain remnant fragments of varying sizes. c–h Quantification of longitudinal neurons in regenerates with shifted amputation sites at time 0 cut, 24hpa, and 7dpa. c Regenerates from an aborally shifted amputation site in large animals regenerated their longitudinal neurons (p = 0.046; F 2,14 = 5.58, p = 0.017, ηp2 = 0.44, n = 8), resembling regenerates of small- and medium-sized animals bisected at the midline of the oral-aboral axis (d). e Regenerates from an orally shifted amputation site in medium animals did not regenerate their longitudinal neurons (p = 1.00; F 2,24 = 21.67, p < 0.001, ηp2 = 0.64, n = 13), resembling regenerates from large animals bisected at the midline of the oral-aboral axis (f). g Regenerates from an orally shifted amputation site in small animals did not regenerate their longitudinal neurons (p = 0.47; F 2,24 = 8.56, p = 0.002, ηp2 = 0.42, n = 13), which is different from medium animals bisected at the midline of the O-A axis (h). See Additional file 15: Table S5 for full model results. Data points in c–h represent means ± SEM, *p ≤ 0.05Back to article page