Fig. 3
From: Characterization of a novel type of carbonic anhydrase that acts without metal cofactors
Point mutations of active site residues. a, b Active sites of all2909 and the 3rd domain of Bn86287. Simulated annealing Fo–Fc omit maps (green) for bicarbonate (BCT) are displayed at a contour level of 3.0 σ. Relevant distances between bicarbonate oxygen atoms/iodide (IOD) and neighbor residues are indicated by dashed lines with numbers. The images were prepared with PyMOL v. 2.3.3 (Schrödinger). c, d CO2 hydration activity of all2909 and the 3rd domain of Bn86287 and their mutants. Mutated residues are indicated by the one-letter code and number. Error bars indicate the SD of triplicate experiments. Significant differences compared to the WT were determined by the two-tailed Student’s t test and indicated by asterisks (P < 0.005)