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Fig. 4 | BMC Biology

Fig. 4

From: Characterization of a novel type of carbonic anhydrase that acts without metal cofactors

Fig. 4

Subcellular localization of Bn86287 and Bn50950. a Immunoblot analysis of an anti-Bn86287 antibody against total proteins of Bigelowiella natans. A mature form of Bn86287 is estimated to be 55–59 kDa. b Confocal images of B. natans cells labeling with the anti-Bn86287 and an FITC-conjugated secondary antibody. Chlorophyll autofluorescence (Chl) is shown in red. c Immungold localization of Bn86287 in the plastid stroma of B. natans. Mt, mitochondrion; N, nucleus; Nm, nucleomorph; P, plastid; Py, pyrenoid. d Confocal images of the chlorarachniophyte Amorphochlora amoebiformis expressing the YFP fusion protein of mitochondrial Bn50950 (upper), and a cell stained by MitoTracker Orange CMTMRos (lower)

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