Fig. 4
From: An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel
The W344R mutation disrupts Kv7.2 interaction with CaM in living cells. a FRET efficiency from spectrally unmixed confocal images of HEK293T cells that express YFP-tagged channels or C-terminal domains (AB, ABCD) and CaM with an N-terminus mCFP-tag for the indicated configurations. There is an additional helix in the linker joining helices A and B referred to as TW. b FRET efficiency between ABCD domains before and after (dashed columns) CaM co-expression. Number of cells is indicated in brackets. ***p < 0.001