Fig. 5
From: An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel
The W344R mutation is compatible with in vitro refolding of the CRD. a Top: Schematic representation of the “folding biosensor”. Helices A and B are flanked by blue mTFP1 and yellow mcpVenus fluorescent proteins. Bottom: Schematic interpretation of the experiment. Upon refolding in the presence of CaM, the fluorescent proteins are within FRET distance. Emission spectra of WT (b) and W344R (c) biosensors after denaturalization with 6 M urea and subsequent dialysis without (solid lines) and with (dashed lines) CaM. d FRET efficiency values of WT (white) and W344R (gray) proteins from data as in B and C. n = 3