Fig. 7
From: An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel
Two orientations for the lateral chain at position 344. a Top: Overall view of the CaM/AB complex with W344 in the N orientation (represented as filled stick, and colored using the Pymol atom coloring scheme). Bottom: Overlaid representation of both tilted (T, red) and native-like (N, green) structures of WT (left) and W344R mutant (right), where hA is blue whereas the CaM C-lobe is orange. Angles between hA and T or N structures are indicated. b Projection of the free energy in the angles between hA and tryptophan or arginine at position 344. Note that the angles of the N and T orientations depend on the presence or absence of CaM. Due to the mathematical computations involved, as WT is not able to explore angles in the ranges (20–65°) and (125–150°), the resulting free energy would be infinite, and it is not showed. The inset shows the time series of the angle through the simulation (Additional file 1: Figure S10)