Fig. 5

Inducible knock-down of PfLPL1 protein in the transgenic parasites and its effect on growth and development asexual cycle of the parasites. Tightly synchronized ring stage parasite culture (0.2% parasitaemia) of transgenic parasites was grown with or without TMP (control and PfLPL1 inducible knock-down, PfLPL1-iKO, respectively). a Flow cytometry histogram showing reduction in population of GFP-labelled parasites in transgenic parasite cultures after PfLPL1 inducible knock-down (grown in absence of TMP) as compared to control parasites (grown in presence of TMP). b Graph showing parasite growth for three asexual cell cycles of the parasite, as determined by counting total parasitaemia at 48, 96, and 144 h. All analyses were performed in triplicate or more (n = 3); the error bars indicate standard deviations. c Graphs showing parasite stage composition at different time points (0–120 h) in parasite culture from control and PfLPL1-iKO sets. d Giemsa-stained images of parasites showing effect on parasite morphology at different time points (0–48 h) in parasite culture control and PfLPL1-iKO sets