Fig. 6

Inducible knock-down of PfLPL1 protein in the transgenic parasites and its effect on the development of neutral lipid body. a, b Synchronous transgenic parasites at ring stages were grown till late trophozoite stages in control and PfLPL1-iKO sets, stained with Nile red and analysed by flow cytometry. Flow cytometry histogram showing concomitant reduction in GFP fluorescence (FL-1) and Nile red labelling (FL-2) in parasites after PfLPL1-iKO as compared to control parasites. c Fluorescence images of trophozoites stage transgenic parasites in PfLPL1-iKO set, showing the reduction in Nile red fluorescence intensity and loss of GFP-fluorescence as compared to control parasite. The parasite nuclei were stained with DAPI (blue) and parasites were visualized by confocal laser scanning microscope