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Fig. 7 | BMC Biology

Fig. 7

From: An essential vesicular-trafficking phospholipase mediates neutral lipid synthesis and contributes to hemozoin formation in Plasmodium falciparum

Fig. 7

Inducible knock-down of PfLPL1 protein in the transgenic parasites and its effect on lipid homeostasis. Synchronous transgenic parasites at ring stages were grown till late trophozoite stages and lipids compositions were assessed by mass spectrometry-based lipidomic analyses from PfLPL1-iKO and control sets. a Analysis of relative amount of total fatty -acids (reflecting the FA composition of existing phospholipids + neutral lipids + free FA pool) in PfLPL1-iKO as compared to control set. b Analysis of fatty acids (FAs) showing no significant changes in the FA composition of parasites from control and PfLPL1-iKO sets. c Analysis of the parasites phospholipid composition showing downregulation of phosphatidic acid (PA) and phsophatidylethanolamine (PE) and upregulation of phosphatidylcholine (PC) and phosphatidylserine (PS) level in the parasite after knock-down of PfLPL1. d Analysis of major neutral lipids levels showing upregulation of diacylglycerols (DAGs) and downregulation of triacylglycerols (TAGs) in PfLPL1-iKO set. All analyses were performed in triplicate (n = 3) or more; the error bars indicate standard deviations. The p values were calculated by Student’s t test: *p < 0.01, **p < 0.005, and ***p < 0.001. PA, phosphatidic acid; PE, phsophatidylethanolamine; PC, phosphatidylcholine; PS, phosphatidylserine; PI, phosphatidylinositol; SM, sphingomyelin; CL, cardiolipin; LPI, lysophosphatidylinositol; and PG, phosphatidylglycerol

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