Fig. 4

Patterns and statistical significance of enrichment of the RAT signal in the co-expressed genes. a Plots showing ANARS for gene bodies and ± 5 kb flanking regions for all genes co-expressed with vlincRNA ID-1132 and the background genes. The sizes of the genic regions were scaled to 5 kb. The ANARS shown in this example was calculated based on the RAT assay performed in the DMSO-treated cells. The ANARS for the positively, negatively and the control background genes is represented by respectively red, blue, and orange dots. b ECDF plots for the data shown in a. Note the shift to the right of the plots corresponding to the co-expressed genes signifying increase in the signal relative to the background genes. The top 30% of the data used for the statistical significance analysis are demarcated by the boxes. c Summary of the distribution of the statistical significance of enrichment of ANARS in the co-expressed vs the background genes (top) and cis vs all genes (bottom). d Plots showing ANARS for gene bodies and ± 5 kb flanking regions for genes co-expressed with vlincRNA ID-1132 (and located on the same chromosome (cis, red dots) and all co-expressed genes (blue dots). The sizes of the genic regions were scaled to 5 kb. e Boxplots of the data presented in d for positions with non-zero ANARS