HAS-7 inhibits HyWnt3 induced ectopic axis in Xenopus laevis axial duplication assay. a, b Xenopus Wnt8 (XWnt8) mRNA injection induces axis duplication in Xenopus embryos compared to uninjected controls. c HyWnt3 mRNA induces axis duplication comparable to XWnt8 mRNA. d Double injection of HyWnt and HAS-7 mRNAs reverts the axis duplication phenotype. e Quantification of embryos showing a double axis phenotype. Injected mRNA doses were as indicated. n indicates the number of embryos analyzed for each experimental condition. Arrows indicate cement glands. Scale bars = 100 μm.