Fig. 1

Su(Hw) binding sites induce bxdPRE silencing activity. a Map of transgenes. Labels: “attB” – attB site required for transgene integration into the attP insertion site; “bxdPRE” – bxdPRE silencing element; “T,” terminators of transcription; white -marker gene; “E” - enhancer of the white gene, “4xSu” – four binding sites for Su(Hw) protein. b The eye phenotypes of flies that have the control “E-w” transgene integrated into the different attP sites: 22A (Bloomington Drosophila Stock Center (BDSC) #24481), 51C (BDSC #24482), 58A (BDSC #24484), 68E (BDSC #24485), 96E (BDSC #24487). P/+, hemizygous; P/P, homozygous adult flies. c The eye phenotypes of flies that have the “bxd” transgene integrated into the different attP sites: 22A, 51C, 58A, 68E, and 96E. d Ph enrichment at bxdPRE in “bxd” transgenes integrated into the different attP insertion sites. Diagrams summarize the results of X-ChIP with Ph antibody or with IgG from a non-immunized animal as a negative control. X-ChIP was analyzed by real-time PCR with primers specific to transgene bxdPRE—the region indicated by underlined number 2 above the “bxd” transgene map. The X-ChIP experiments were performed with chromatin isolated from the heads of homozygous adult flies. The ordinate shows the percentage of target sequences in the immunoprecipitated material relative to the input DNA and normalized to the positive control—a sequence adjacent to the endogenous bxdPRE in BX-C (bxdPRE-Genome). The line with bxd transgene is designated by its attP insertion site and is indicated on the abscissa. Vertical lines indicate SDs. e The eye phenotypes of flies with the “bxd,” “Su-bxd,” or “Su” transgenes at the 96E attP site