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Fig. 2 | BMC Biology

Fig. 2

From: Boundaries potentiate polycomb response element-mediated silencing

Fig. 2

Su(Hw) boundary induces the recruitment of PcG proteins to the bxdPRE. (a) Maps of the “bxd” and “Su-bxd” transgene constructs. The numbers above the maps (1, 2, 3, 4, 5, 6) indicate the regions amplified by qPCR in X-ChIP experiments. The X- ChIPs were performed with chromatin isolated from heads of adult flies homozygous for the “bxd” or “Su-bxd” transgenes at 96E insertion site. The X-ChIPs were performed with specific antibodies or with IgG. The specific antibodies: (b) Ph, (c) Sfmbt, (d) Pho, (e) Combgap, (f) Trx, (g) CBP. The ordinate shows the percentage of target sequences in the immunoprecipitated material relative to the input DNA and normalized to bxdPRE-Genome. The transgene specific regions and negative genome control (ras - coding part of Ras64B gene) are indicated on the abscissa. Other designations are as in Fig. 1

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