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Fig. 5 | BMC Biology

Fig. 5

From: Boundaries potentiate polycomb response element-mediated silencing

Fig. 5

Su(Hw) multimer can induce enPRE silencing. a Map of “en” and “Su-en” transgenes. Designations: “en”—enPRE element. Other labels are as in Fig. 1. b The eye phenotypes of flies with “en” and “Su-en” transgenes integrated into different attP insertion sites. c The results of X-ChIP with chromatin isolated from adult heads of homozygous lines with “en” or “Su-en” transgenes integrated into different attP insertion sites. X-ChIP was performed with Ph and Sfmbt antibodies or with an IgG control followed by real-time PCR with primers specific to transgenic enPRE. The ordinate shows the percentage of target sequences in the immunoprecipitated material relative to the input DNA and normalized relative to bxdPRE-Genome. The lines with enPRE transgene are designated by the attP insertion site as indicated on the abscissa. Other designations are as in Fig. 2

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