Skip to main content
Fig. 6 | BMC Biology

Fig. 6

From: Boundaries potentiate polycomb response element-mediated silencing

Fig. 6

Impact of distance between the boundary and the bxdPRE on PcG-dependent silencing. All transgenes were integrated in 96E insertion site. a Su-1kb-bxd, Su-3kb-bxd—the 4xSu sites are placed at the distance of 1 or 3 kb from the bxdPRE. CTCF-1kb-bxd, CTCF-3kb-bxd—the 4xCTCF sites are placed at the distance of 1 or 3 kb from the bxdPRE. Eye phenotypes in hemizygous (P/+) and homozygous (P/P) flies are shown. b The phenotypes of trans-heterozygotes. To generate the Su-1kb-bxd/CTCF-1kb-bxd or Su-3kb-bxd/CTCF-3kb-bxd trans-heterozygotes, Su-1kb-bxd and CTCF-1kb-bxd or Su-3kb-bxd and CTCF-3kb-bxd homozygous flies were crossed with each other. Hypothetical models of interactions in hemizygotes (c), homozygotes, and trans-heterozygotes (d) are shown. Red ovals—enhancer-associated activator proteins; blue and pink ovals—Polycomb and Trithorax group proteins, respectively

Back to article page