Fig. 2

Characterization of EVT by transmission electron microscopy. a, b Cells of the indicated genotypes were treated as in Fig. 1a. At 32 h after dilution to OD₆₀₀=0.2, cells were collected and processed for TEM. Multiple vesicles accumulated in the vacuoles of pep4Δ, atg15Δ, atg8Δ, and hfl1Δ cells. The lumenal vesicles in atg8Δ and hfl1Δ cells were generally larger than those in pep4Δ and atg15Δ cells. For each strain, two representative cells are presented (a). Scale bar, 1 μm. For each strain that contained a substantial amount of lumenal structures, the diameters of approximately 200 vesicle-like structures were measured. The resulting distributions are presented in b