Fig. 3
CeA glutamatergic projections course within the lateroventral portion of the PnC. a Left, Sagittal representation of the mouse brain illustrating the AAV-CamKIIα-eYFP injection site targeting CeA neurons (green circle) and CeA projection fibers terminating at the level of the PnC (red circle). The dotted line illustrates the PnC level at which coronal cut sections were obtained to visualize CamKIIα-eYFP+ axons originating from the CeA. Right, Schematic of the hypothesis being tested. b Representative CeA coronal sections showing eYFP+ fluorescence (green) and NeuroTraceTM staining (magenta). The white rectangle shows the area imaged in panels c–e. Inset, Nissl stain image of the injection site in the CeA. Arrowheads represent the injection needle tract, dorsal to imaging site. The black square corresponds to the white square area on the fluorescence image. c White arrows indicate CeA cells positive for CamKIIα-eYFP (green). d NeuroTraceTM stain (magenta) labels CeA cells bodies (white arrows). e White arrows indicate CeA cells positive for CamKIIα-eYFP and NeuroTraceTM. f Representative image of CamKIIα-eYFP+ CeA fibers (green) coursing within a PnC coronal section stained with NeuroTraceTM (magenta). The three arrowheads indicate the 7th cranial nerve. Inset, lower magnification of the PnC coronal section with PnC delineated landmarks. The arrowhead indicate the location of the track left by the implanted optic fiber for PPI in vivo experiments. Representative of N = 4 mice. Scale bars: c, e 400 μm, d–f 50 μm