Fig. 7

CamKIIα⁺ CeA fibers closely apposed to GlyT2⁺ PnC neurons. a Top, Schematic of the hypothesis being tested. Bottom, Orthogonal view of a close apposition between CamKIIα-mCherry⁺ CeA excitatory fibers (magenta) and the soma of a GlyT2⁺ PnC neuron (green) indicated by the arrowhead in all three views. b Three-dimensional reconstruction of putative synaptic contacts between CamKIIα-mCherry⁺ CeA fibers (magenta) and GlyT2-eGFP⁺ neurons expressing PSD-95 (blue; arrow). Few putative synaptic appositions did not show PSD-95 staining (arrowheads). c Volume rendering and angular sectioning of the PSD-95⁺ putative synaptic contact shown in b. Representative of N = 6 mice. Scale bar in a is 50 μm