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Fig. 3 | BMC Biology

Fig. 3

From: Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs

Fig. 3

Selection of alkyne-adapters through sequencing of miRXplore Universal Reference RNA libraries. a A schematic of the mDOT-seq. b The majority of miRNAs are detected as full-length sequences in all libraries. Bar plot shows the observed fraction of miRNAs after increasing number of nucleotides is cropped from its 3′ end. The exact numbers are provided in Additional file 1: Table S1. c 2C and 2U libraries contain the highest portion of accurately quantified miRNA reads. miRNAs identified in the reference set of sequences cropped of two nt at their 3′ end and having at least 1 CPM in each library were used to calculate fold-deviation from the equimolar input and plotted as log2 values. Values within two folds from the expected ones (grey vertical lines) were considered unbiased according to [27]. d Top 30 of 2C or 2U 3′ adapters containing different bases at first, third and fourth 5′ positions with the highest number of identified reads. The libraries were demultiplexed using each of 3′ adapters and the total number of miRNA reads was calculated. Sequence logos of top ranked adapters were created using WEBLOGO [28]. The data underlying the presented graphs are in Additional file 2

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