Table 1 Overview of used antibodies and markers
| Name | Abbreviation | Host species | Dilution | Vendor/donor (catalogue #, batch #, RRID/CAS #) | References | Specificity |
|---|---|---|---|---|---|---|
| 5-Ethynyl-2′-desoxuridine | EdU | 100 μM |
Thermo Fischer Scientific, Rockford, IL, USA (A10044; 1259424; 61135-33-9) | [122, 123] | ||
| Alexa Fluor 488-coupled phalloidin | Phalloidin | 1:200 |
Thermo Fischer Scientific, Rockford, IL, USA (A12379; n/a; n/a) | [124] | ||
| Alexa Fluor 488 Azide | 488-azide | 1 μM |
Thermo Fischer Scientific, Rockford, IL, USA (A10260; 1320994; n/a) | |||
| Cy2-coupled donkey anti-sheep | DAS-Cy2 | Donkey | 1:300 |
Jackson ImmunoResearch; Westgrove, PA, USA (713-225-147, n/a, AB_2340735) | ||
| Cy3-coupled goat anti-chicken | GACh-Cy3 | Goat | 1:300 |
Jackson ImmunoResearch; Westgrove, PA, USA (103-165-155, 93117 / 139580, AB_2337386) | ||
| Cy3-coupled goat anti-rabbit | GAR-Cy3 | Goat | 1:300 |
Jackson ImmunoResearch; Westgrove, PA, USA (111-165-144, n/a, AB_2338006) | ||
| Cy5-coupled donkey anti-mouse | DAS-Cy5 | Donkey | 1:300 |
Jackson ImmunoResearch; Westgrove, PA, USA (715-005-150,132236, RB_2340758) | ||
| Cy5-coupled goat anti-mouse | GAM-Cy5 | Goat | 1:300 |
Jackson ImmunoResearch; Westgrove, PA, USA (115-175-146, n/a, AB_2338713) | ||
| Cy5-Sulfo Azide | Cy5-azide | 1 μM |
Jena Bioscience, Jena, Germany (CLK-AZ118-1; Kli009-030; n/a) | |||
|
Diploptera punctata Allatostatin I | Dip-AST | Rabbit | 1:20,000 |
H.J. Agricola (Friedrich Schiller University, Jena, Germany) (n/a, n/a, AB_2314318) | [125] | Ame: [71] |
|
Drosophila melanogaster Synapsin I (SYNORF1) | Synapsin | Mouse | 1:50 |
E. Bucher, University of Würzburg, Germany (n/a, n/a, AB_2313617) | [126] |
Ame: [127] Tcas: [113] |
| HRP-coupled donkey anti-sheep | DAS-HRP | Donkey | 1:1,000 |
Jackson ImmunoResearch; Westgrove, PA, USA (713-035-147, 69205, AB_2340710) | ||
| HRP-coupled goat anti-rabbit | GAR-HRP | Goat | 1:1,000 |
Jackson ImmunoResearch; Westgrove, PA, USA (111-035-003, 130223, AB_2313567) | ||
| Moth-R2, Orco antiserum | Moth-R2 | Rabbit | 1:5,000 |
J. Krieger, University Halle-Wittenberg, Germany (n/a; n/a; n/a) | [5] | Tcas: [5] |
| Rattus norvegicus glutamate decarboxylase (rabbit) | GADr | Rabbit | 1:1,000 |
Sigma-Aldrich; now Merck KGaA, Darmstadt, Germany (G5163; 113M4772; AB_477019) | Tcas: This study by Western blot | |
| Rattus norvegicus glutamate decarboxylase (sheep) | GADs | Sheep | 1:5,000 |
W. Oertel, Laboratory of Clinical Science, Mansfield, MA, USA (n/a; n/a; AB_2314497) | [128] | Tcas: This study by Western blot |
| Red fluorescent protein | RFP | Chicken | 1:3,000 |
Rockland Immunochemicals INC, Limerick, PA, USA (600-901-379, 26274, AB_10704808) |