Fig. 6

Functional characterization of iRP-D26 and iRP-D28A. a Representative micrographs of iRP-D26 and iRP-D28A trans-well inserts after migration and Matrigel invasion assays. Nuclei were counterstained with DAPI (blue). b Box plot of average cell counts (n=5) from uncoated (migration, n=3) and Matrigel-coated (invasion, n=3) inserts under normoxic (blue) and hypoxic (red) conditions. A two-sided unpaired t test with alpha of 0.05 was used to determine significance. c Box plot of percent invasion (ratio of invasive cells relative to migratory cells) determined for each of the cell lines under normoxic (blue) and hypoxic (red) conditions. d Bar chart of IGF2 qRT-PCR expression levels of iRFb (n=2) and iRP samples (n=4). e Bar chart of IGF2 protein secretion levels for iRFb (n=2), iRP (n=3), and the rhesus serum pool (n=4); error bars depict standard error (SE)