Fig. 9
TRL4 and an autophagy-based mechanism regulate NPM secretion in response to Dox. a hCmPCs were pre-treated with 100 ÎĽM TLR4i for 30 min. Then 1 ÎĽM Dox was added to cells for further 8 h. Afterwards, serum-free media were collected and analysed by ELISA assay for the presence of eNPM. As already observed, Dox induced a significant secretion of NPM vs Ctrl while the inhibition of TLR4 inhibited NPM secretion in the extracellular milieu (n = 4; *P < 0.05). b hCmPCs were pre-treated with two autophagy inhibitors, 50 ÎĽM chloroquine (Chl) and 5ÎĽM 3-MA (5) for 30 min, then 1 ÎĽM Dox was added to cells for further 8 h. Serum-free media were collected and analysed by ELISA assay for the presence of eNPM. Dox induced a significant secretion of NPM vs Ctrl while the treatment with Chl and 3-MA significantly inhibited NPM secretion in the extracellular milieu (n = 7; *P < 0.05)