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Fig. 9 | BMC Biology

Fig. 9

From: Doxorubicin induces an alarmin-like TLR4-dependent autocrine/paracrine action of Nucleophosmin in human cardiac mesenchymal progenitor cells

Fig. 9

TRL4 and an autophagy-based mechanism regulate NPM secretion in response to Dox. a hCmPCs were pre-treated with 100 μM TLR4i for 30 min. Then 1 μM Dox was added to cells for further 8 h. Afterwards, serum-free media were collected and analysed by ELISA assay for the presence of eNPM. As already observed, Dox induced a significant secretion of NPM vs Ctrl while the inhibition of TLR4 inhibited NPM secretion in the extracellular milieu (n = 4; *P < 0.05). b hCmPCs were pre-treated with two autophagy inhibitors, 50 μM chloroquine (Chl) and 5μM 3-MA (5) for 30 min, then 1 μM Dox was added to cells for further 8 h. Serum-free media were collected and analysed by ELISA assay for the presence of eNPM. Dox induced a significant secretion of NPM vs Ctrl while the treatment with Chl and 3-MA significantly inhibited NPM secretion in the extracellular milieu (n = 7; *P < 0.05)

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